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his talon gravity column  (TaKaRa)


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    Structured Review

    TaKaRa his talon gravity column
    His Talon Gravity Column, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/disposable+gravity+column/TALON+Disposable+Gravity+Column/pmc13110175-185-6-10
    Average 99 stars, based on 92 article reviews
    his talon gravity column - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Kit for detecting antigen or measuring its amount
    Article Snippet: .. Addition of 500 μL of PB, centrifugation, and removal of the supernatant are repeated three times to wash the beads; 5) Add the beads in step 4) to the supernatant in step 3) and rotate with a mini rotary mixer NRC-20D (NISSIN) at room temperature for 1 hour; 6) Centrifuge at 4° C. at 100×g for 1 minute and remove the supernatant; 7) Transfer the beads to TALON (registered trademark) 2-mL Disposable Gravity Column (Takara Bio Inc.) and discharge the supernatant; 8) Add 500 μL of His Spin Trap binding buffer three times to wash the beads; and 9) Add 200 μL of His Spin Trap elution buffer to elute the target protein and collect the protein in a 1.5-mL tube. ..

    Binding Assay:

    Article Title: Kit for detecting antigen or measuring its amount
    Article Snippet: .. Addition of 500 μL of PB, centrifugation, and removal of the supernatant are repeated three times to wash the beads; 5) Add the beads in step 4) to the supernatant in step 3) and rotate with a mini rotary mixer NRC-20D (NISSIN) at room temperature for 1 hour; 6) Centrifuge at 4° C. at 100×g for 1 minute and remove the supernatant; 7) Transfer the beads to TALON (registered trademark) 2-mL Disposable Gravity Column (Takara Bio Inc.) and discharge the supernatant; 8) Add 500 μL of His Spin Trap binding buffer three times to wash the beads; and 9) Add 200 μL of His Spin Trap elution buffer to elute the target protein and collect the protein in a 1.5-mL tube. ..

    Purification:

    Article Title: Cis-regulatory elements and transcription factors related to auxin signaling in the streptophyte algae Klebsormidium nitens
    Article Snippet: .. The supernatant was collected in another tube and used for His-tag purification with His60 Ni Superflow (Clontech) and a TALON 2 mL Disposable Gravity Column (TaKaRa) in accordance with the instruction manual. ..

    Article Title: Cis-regulatory elements and transcription factors related to auxin signaling in the streptophyte algae Klebsormidium nitens.
    Article Snippet: .. The supernatant was collected in another tube and used for His-tag purification with His60 Ni Superflow (Clontech) and a TALON 2 mL Disposable Gravity Column (TaKaRa) in accordance with the instruction manual. ..

    Article Title: Impaired 8-Hydroxyguanine Repair Activity of MUTYH Variant p.Arg109Trp Found in a Japanese Patient with Early-Onset Colorectal Cancer
    Article Snippet: E. coli BL21-CodonPlus (DE3)-RP competent cells (Stratagene) were transformed with the MUTYH-pET25b vector and cultured at 37°C until an A 600 of 0.6. .. After incubation with 0.1 mM IPTG at 15°C for 12 h, MUTYH-His 6 protein was purified with TALON metal affinity resins (Clontech, Palo Alto, CA) and a TALON 2 mL disposable gravity column (Clontech). ..

    Incubation:

    Article Title: Impaired 8-Hydroxyguanine Repair Activity of MUTYH Variant p.Arg109Trp Found in a Japanese Patient with Early-Onset Colorectal Cancer
    Article Snippet: E. coli BL21-CodonPlus (DE3)-RP competent cells (Stratagene) were transformed with the MUTYH-pET25b vector and cultured at 37°C until an A 600 of 0.6. .. After incubation with 0.1 mM IPTG at 15°C for 12 h, MUTYH-His 6 protein was purified with TALON metal affinity resins (Clontech, Palo Alto, CA) and a TALON 2 mL disposable gravity column (Clontech). ..

    other:

    Article Title: Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing.
    Article Snippet: Protein was concentrated using an Amicon 100-kDa centrifugal filter (Sigma, UFC810024) and buffer-exchanged into storage buffer (600 mM NaCl, 50 mM Tris-HCl pH 7.5, 5% glycerol and 2 mM DTT), quantified by Bolt Bis-Tris Plus 4–12% gels (Thermo Fisher, NW04120BOX) in 1× MES SDS running buffer (Thermo Fisher, B0002) with a PageRulerPlus Prestained Protein Ladder (Thermo Fisher, 26619), aliquoted and frozen at −80 °C for storage.



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